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Sajeev, T. V.
- A New Insect Rearing Container for in vivo Mass Multiplication of NPV of Hyblaea puera
Abstract Views :202 |
PDF Views:114
Authors
Affiliations
1 Division of Forest Protection, Kerala Forest Research Institute, Peechi - 680653, Thrissur, Kerala, IN
1 Division of Forest Protection, Kerala Forest Research Institute, Peechi - 680653, Thrissur, Kerala, IN
Source
Journal of Biological Control, Vol 22, No 1 (2008), Pagination: 217-219Abstract
To overcome the problems in rearing the teak defoliator, Hyblaea puera on artificial diet in a two-piece rearing tube for HpNPV mass multiplication, a new three-piece tube was designed and evaluated. The two-piece tube (Tube A) consists of two parts - a cylindrical main body with one end open (55mm x 23mm) and a perforated cap. The three-piece tube (Tube B) consists of a main body with both ends open (55mm % 23mm), a diet cup (20mm % 22mm) and a perforated cap. Comparative evaluation of two types of tubes (main body of Tube A vs Diet cup of Tube B) was made in terms of their virus capture efficiency during inoculation and also easiness in diet dispensing and general handling. The short length of the diet cup of tube B favored highest virus capturing (added 30%) and efficient diet dispensing. Tube B could be an excellent option for rearing H. puera larvae for NPV mass production and laboratory culture maintenance and also for rearing of other lepidopteran larvae of similar behavior and size.Keywords
HpNPV, Hyblaea puera, Rearing Tube.- An Improved Method of Extraction of Nucleopolyhedrovirus from Soil with Reference to Hyblaea puera NPV
Abstract Views :171 |
PDF Views:107
Authors
Affiliations
1 Forest Protection Division, Kerala Forest Research Institute, Peeehi, 680653, Kerala, IN
1 Forest Protection Division, Kerala Forest Research Institute, Peeehi, 680653, Kerala, IN
Source
Journal of Biological Control, Vol 22, No 2 (2008), Pagination: 479-481Abstract
An efficient method of extraction of the nucleopolyhedrovirus of the teak defoliator, Hyblaea puera (Cramer) (Lepidoptera: Hyblaeidae) (HpNPV) from soil has been standardized. This method ensures recovery of about 34 per cent of NPV from soil.Keywords
Extraction, Hyblaea puera, HpNPV, Nucleopolyhedrovirus, Persistence, Soil.- Economics of HpNPV Production Using Field Collected and Laboratory Reared Hyblaea puera (Cramer) (Lepidoptera: Hyblaeidae)
Abstract Views :279 |
PDF Views:125
Authors
Affiliations
1 Forest Protection Programme Division, Kerala Forest Research Institute, Peechi, 680 653, Trichur, Kerala, IN
1 Forest Protection Programme Division, Kerala Forest Research Institute, Peechi, 680 653, Trichur, Kerala, IN
Source
Journal of Biological Control, Vol 19, No 2 (2005), Pagination: 193-196Abstract
The economics of mass production of the nucleopolyhedrovirus of the teak defoliator, Hyblaea puera Cramer (Lepidoptera: Hyblaeidae) (HpNPV) using the laboratory-reared and field-collected (from teak plantations) host larvae was compared. The data were based on 7645 laboratory-reared (LR) larvae and 8925 field-collected (FC) larvae. The virus production was carried out using fifth instar larva inoculated with 1x106 OBs (Viral Occlusion Bodies)/larva. The virus yield/larva in LR and FC larvae was 4.8x108 and 3.6x108 OBs, respectively. The virus productivity ratio in LR and FC larvae was 241 ± 55 and 178 ± 100.85 OBs, respectively. The cost of production of HpNPV per larva was higher in the case of LR (Rs. 0.82/larva) in comparison with FC (Rs.0.72). However, the cost of HpNPV required for spraying in one hectare at the rate of 1.63 x 1011 OBs worked out to be Rs. 279 and Rs. 317 in the case of LR and FC, respectively. A marginal difference in the virus yield was found affecting the cost of the virus produced.Keywords
Hyblaea puera, HpNPV, Mass Production, Nucleopolyhedrovirus, Teak Defoliator.- Mass Production of Nucleopolyhedrovirus of the Teak Defoliator, Hyblaea puera Cramer Using Host Population in Teak Plantations
Abstract Views :231 |
PDF Views:149
Authors
Affiliations
1 Division of Entomology, Kerala Forest Research Institute, Peechi 680653, Kerala, IN
1 Division of Entomology, Kerala Forest Research Institute, Peechi 680653, Kerala, IN
Source
Journal of Biological Control, Vol 18, No 1 (2004), Pagination: 81-84Abstract
The nucleopolyhedrovirus of the teak defoliator, Hyblaea puera Cramer (Lepidoptera: Hyblaeidae) (HpNPV) is a potential biocontrol agent of the pest. This paper describes a method to mass multiply HpNPV in situ in the field population of H. puera. The method is based on application of the virus suspension on young teak plantation infested with H. puera. An average yield of 4.8816 x 10& Polyhedral Occlusion Bodies (POBs) per fifth instar larva was obtained with this method. The advantage of this method of HpNPV production is that it is less expensive as it does not depend on host larval culture maintained in the laboratory.Keywords
Hyblaea puera, HpNPV, Mass Production, Nucleopolyhedrovirus, Teak Defoliator.- Field Efficacy of Nuclear Polyhedrosis Virus for Protection of Teak against the Defoliator, Hyblaea puera Cramer (Lepidoptera:Hyblaeidae)
Abstract Views :229 |
PDF Views:141
Authors
K. S. S. Nair
1,
Biju Babjan
1,
T. V. Sajeev
1,
V. V. Sudheendrakumar
1,
M. I. Mohammed Ali
2,
R. V. Varma
1,
K. Mohanadas
1
Affiliations
1 Division of Entomology, Kerala Forest Research Institute, Peechi 680 653, Kerala, IN
2 Division of Plant Pathology, Kerala Forest Research Institute, Peechi, IN
1 Division of Entomology, Kerala Forest Research Institute, Peechi 680 653, Kerala, IN
2 Division of Plant Pathology, Kerala Forest Research Institute, Peechi, IN